Recombinant humaan RNA polymerase I en III subeenheid RPAC1/POLR1C eiwit

POLR1C is a subunit of both RNA polymerase I and RNA polymerase III complexes. The encoded protein is part of the Pol core element. Defects in this gene have been associated with Treacher Collins syndrome (TCS) . Recombinant human POLR1C protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

DNA-directed RNA polymerases I and III subunit RPAC1, RP3-337H4.4, RPA39, RPA40, RPA5, RPAC1, TCS3

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 80% by SDS-PAGE

41.6 kDa (369aa) confirmed by MALDI-TOF

POLR1C, DNA-directed RNA polymerases I and III subunit RPAC1, RP3-337H4.4, RPA39, RPA40, RPA5, RPAC1, TCS3, ATGP2753-10 µg, ATGP2753-20 µg, ATGP2753-50 µg, ATGP2753-100 µg, ATGP2753-250 µg, ATGP2753-500 µg, ATGP2753-1 mg, ATGP2753-10, ATGP2753-20, ATGP2753-50, ATGP2753-100, ATGP2753-250, ATGP2753-500, ATGP2753-1

Hu P., et al (2002) . Mol. Cell. Biol. 22:8044-8055; ; Dauwerse J.G., et al (2011) . Nat. Genet. 43:20-22;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT

Epigenomics (Transcription & Translation)

NP_976035

O15160

Human

MGSSHHHHHH SSGLVPRGSH MGSMAASQAV EEMRSRVVLG EFGVRNVHTT DFPGNYSGYD DAWDQDRFEK NFRVDVVHMD ENSLEFDMVG IDAAIANAFR RILLAEVPTM AVEKVLVYNN TSIVQDEILA HRLGLIPIHA DPRLFEYRNQ GDEEGTEIDT LQFRLQVRCT RNPHAAKDSS DPNELYVNHK VYTRHMTWIP LGNQADLFPE GTIRPVHDDI LIAQLRPGQE IDLLMHCVKG IGKDHAKFSP VATASYRLLP DITLLEPVEG EAAEELSRCF SPGVIEVQEV QGKKVARVAN PRLDTFSREI FRNEKLKKVV RLARVRDHYI FSVESTGVLP PDVLVSEAIK VLMGKCRRFL DELDAVQMD