Recombinant humaan RNA polymerase I, II en III subeenheid RPABC1/POLR2E eiwit

POLR2E, also as known as DNA-directed RNA polymerases I, II, and III subunit RPABC1, belongs to the archaeal RpoH/eukaryotic RPB5 RNA polymerase subunit family. POLR2E is the fifth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit is shared by the other two DNA-directed RNA polymerases and is present in two-fold molar excess over the other polymerase subunits. POLR2E is a DNA-dependent RNA polymerase that catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Recombinant human POLR2E protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

RNA polymerases I, II, and III subunit RPABC1/POLR2E protein

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

27 kDa (233aa) confirmed by MALDI-TOF

POLR2E, DNA-directed RNA polymerases I II and III subunit RPABC1, DNA-directed RNA polymerases I, II, and III subunit RPABC1, hRPB25, hsRPB5, RPABC1, RPB5, XAP4, ATGP1843-10 µg, ATGP1843-20 µg, ATGP1843-50 µg, ATGP1843-100 µg, ATGP1843-250 µg, ATGP1843-500 µg, ATGP1843-1 mg, ATGP1843-10, ATGP1843-20, ATGP1843-50, ATGP1843-100, ATGP1843-250, ATGP1843-500, ATGP1843-1

Kershnar E., et al. (1998) J. Biol. Chem. 273:34444-34453; ; Dorjsuren D., et al. (1998) Mol. Cell. Biol. 18:7546-7555;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol, 1mM DTT

Epigenomics (Transcription & Translation)

NP_002686

P19388

Human

MDDEEET YRLWKIRKTI MQLCHDRGYL VTQDELDQTL EEFKAQFGDK PSEGRPRRTD LTVLVAHNDD PTDQMFVFFP EEPKVGIKTI KVYCQRMQEE NITRALIVVQ QGMTPSAKQS LVDMAPKYIL EQFLQQELLI NITEHELVPE HVVMTKEEVT ELLARYKLRE NQLPRIQAGD PVARYFGIKR GQVVKIIRPS ETAGRYITYR LVQ