Recombinant humaan RNA polymerase I, II en III subeenheid RPABC3/POLR2H eiwit

POLR2H is one of the essential subunits of RNA polymerase II that is shared by the other two eukaryotic DNA-directed RNA polymerases, I and III. Recombinant human POLR2H protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

DNA-directed RNA polymerases I II and III subunit RPABC3, DNA-directed RNA polymerases I, II, and III subunit RPABC3, hsRPB8, RPABC3, RPB17, RPB8

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

19.5 kDa (173aa) confirmed by MALDI-TOF

POLR2H, DNA-directed RNA polymerases I II and III subunit RPABC3, DNA-directed RNA polymerases I, II, and III subunit RPABC3, hsRPB8, RPABC3, RPB17, RPB8, ATGP2071-10 µg, ATGP2071-20 µg, ATGP2071-50 µg, ATGP2071-100 µg, ATGP2071-250 µg, ATGP2071-500 µg, ATGP2071-1 mg, ATGP2071-10, ATGP2071-20, ATGP2071-50, ATGP2071-100, ATGP2071-250, ATGP2071-500, ATGP2071-1

Kang, X., et al. (2006) J. Biol. Chem. 281 (26), 18216-18226

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Epigenomics (Transcription & Translation)

NP_006223

P52434

Human

MAGILFE DIFDVKDIDP EGKKFDRVSR LHCESESFKM DLILDVNIQI YPVDLGDKFR LVIASTLYED GTLDDGEYNP TDDRPSRADQ FEYVMYGKVY RIEGDETSTE AATRLSAYVS YGGLLMRLQG DANNLHGFEV DSRVYLLMKK LAF