Recombinant humaan RNA polymerase I, II en III subeenheid RPABC4/POLR2K-eiwit

POLR2K is one of the smallest subunits of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit is shared by the other two DNA-directed RNA polymerases. Recombinant human POLR2K protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

DNA-directed RNA polymerases I II and III subunit RPABC4, DNA-directed RNA polymerases I, II, and III subunit RPABC4, ABC10-alpha, hRPB7.0, hsRPB10a, RPABC4, RPB10alpha, RPB12, RPB7.0

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

9.4 kDa (81aa) confirmed by MALDI-TOF

POLR2K, DNA-directed RNA polymerases I II and III subunit RPABC4, DNA-directed RNA polymerases I, II, and III subunit RPABC4, ABC10-alpha, hRPB7.0, hsRPB10a, RPABC4, RPB10alpha, RPB12, RPB7.0, ATGP2892-10 µg, ATGP2892-20 µg, ATGP2892-50 µg, ATGP2892-100 µg, ATGP2892-250 µg, ATGP2892-500 µg, ATGP2892-1 mg, ATGP2892-10, ATGP2892-20, ATGP2892-50, ATGP2892-100, ATGP2892-250, ATGP2892-500, ATGP2892-1

Shpakovski G.V., et al. (1995) Mol. Cell. Biol. 15:4702-4710

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol, 1mM DTT

Epigenomics (Transcription & Translation)

NP_005025

P53803

Human

MDTQKDV QPPKQQPMIY ICGECHTENE IKSRDPIRCR ECGYRIMYKK RTKRLVVFDA R