Recombinant humaan RNA polymerase II subeenheid RPB11-a/POLR2J-eiwit

POLR2J is a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This protein exists as a heterodimer with another polymerase subunit; together they form a core subassembly unit of the polymerase. Two similar genes are located nearby on chromosome 7q22. 1 and a pseudogene is found on chromosome 7p13. Recombinant human POLR2J protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

DNA-directed RNA polymerase II subunit RPB11-a, hRPB14, POLR2J1, RPB11, RPB11A, RPB11m

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

15.7 kDa (140aa) confirmed by MALDI-TOF

POLR2J, DNA-directed RNA polymerase II subunit RPB11-a, hRPB14, POLR2J1, RPB11, RPB11A, RPB11m, ATGP1774-10 µg, ATGP1774-20 µg, ATGP1774-50 µg, ATGP1774-100 µg, ATGP1774-250 µg, ATGP1774-500 µg, ATGP1774-1 mg, ATGP1774-10, ATGP1774-20, ATGP1774-50, ATGP1774-100, ATGP1774-250, ATGP1774-500, ATGP1774-1

Proshkin, S.A., et al. (2011) Biochemistry Mosc. 76 (8), 976-980; ; Shpakovskii, D.G., et al. (2004) Bioorg. Khim. 30 (6), 621-625

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 40% glycerol, 5mM DTT, and 2mM EDTA

Epigenomics (Transcription & Translation)

NP_006225

P52435

Human

MNAPPAF ESFLLFEGEK KITINKDTKV PNACLFTINK EDHTLGNIIK SQLLKDPQVL FAGYKVPHPL EHKIIIRVQT TPDYSPQEAF TNAITDLISE LSLLEERFRV AIKDKQEGIE