Recombinant humaan RNA polymerase II subeenheid RPB11-b1/POLR2J2-eiwit

POLR2J2 is a member of the RNA polymerase II subunit 11 gene family, which includes three genes in a cluster on chromosome 7q22. 1 and a pseudogene on chromosome 7p13. The founding member of family, DNA directed RNA polymerase II polypeptide J, has been shown to encode a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This locus produces multiple, alternatively spliced transcripts that potentially express isoforms with distinct C-termini compared to DNA directed RNA polymerase II polypeptide J. Most or all variants are spliced to include additional non-coding exons at the 3' end which makes them candidates for nonsense-mediated decay (NMD) . Consequently, it is not known if this locus expresses a protein or proteins in vivo. Recombinant human POLR2J2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

DNA-directed RNA polymerase II subunit RPB11-b1, HRPB11B, RPB11b1

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

15.5 kDa (138aa) confirmed by MALDI-TOF

POLR2J2, DNA-directed RNA polymerase II subunit RPB11-b1, HRPB11B, RPB11b1, ATGP1952-10 µg, ATGP1952-20 µg, ATGP1952-50 µg, ATGP1952-100 µg, ATGP1952-250 µg, ATGP1952-500 µg, ATGP1952-1 mg, ATGP1952-10, ATGP1952-20, ATGP1952-50, ATGP1952-100, ATGP1952-250, ATGP1952-500, ATGP1952-1

Shpakovskii, D.G., et al. (2004) Bioorg. Khim. 30 (6), 621-625; ; Benga, W.J., et al. (2005) Nucleic Acids Res. 33 (11), 3582-3590

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT, 250 mM Imidazole

Epigenomics (Transcription & Translation)

NP_116581

Q9GZM3

Human

MGSSHHHHHH SSGLVPRGSH MGSMNAPPAF ESFLLFEGEK ITINKDTKVP KACLFTINKE DHTLGNIIKS QLLKDPQVLF AGYKVPHPLE HKIIIRVQTT PDYSPQEAFT NAITDLISEL SLLEERFRTC LLPLRLLP