Recombinant humaan RNA polymerase II subeenheid RPB11-b2/POLR2J3-eiwit

DNA-directed RNA polymerase II subunit RPB11-b2, also known as POLR2J3, belongs to the eukaryotic RPB11 RNA polymerase subunit family. POLR2J (DNA-directed RNA polymerase II subunit J) exist as three variants POLR2J1 (RPB11-a), POLR2J2 (RPB11-b1), and POLR2J3 (RPB11-b2) . POLR2J3 is DNA-dependent RNA polymerases that catalyze the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Recombinant human POLR2J3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

DNA-directed RNA polymerase II subunit RPB11-b2, POLR2J2, RPB11b1, RPB11b2

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

15.5 kDa (138aa) confirmed by MALDI-TOF

POLR2J3, DNA-directed RNA polymerase II subunit RPB11-b2, POLR2J2, RPB11b1, RPB11b2, ATGP1743-10 µg, ATGP1743-20 µg, ATGP1743-50 µg, ATGP1743-100 µg, ATGP1743-250 µg, ATGP1743-500 µg, ATGP1743-1 mg, ATGP1743-10, ATGP1743-20, ATGP1743-50, ATGP1743-100, ATGP1743-250, ATGP1743-500, ATGP1743-1

Palangat M., et al. (2004) J Mol Biol. 341:429-442; ; uivari A., et al. (2006) Nat Struct Mol Biol. 13: 49-54.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 40% glycerol, 1mM DTT

Epigenomics (Transcription & Translation)

NP_001091084

A0A0B4J2F8

Human

MNAPPAF ESFLLFEGEK ITINKDTKVP NACLFTINKE DHTLGNIIKS QLLKDPQVLF AGYKVPHPLE HKIIIRVQTT PDYSPQEAFT NAITDLISEL SLLEERFRTC LLPLRLLP