Recombinant humaan RNA polymerase II subeenheid RPB3/POLR2C-eiwit

POLR2C is the third largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a cysteine rich region and exists as a heterodimer with another polymerase subunit, POLR2J. These two subunits form a core subassembly unit of the polymerase. A pseudogene has been identified on chromosome 21. Recombinant human POLR2C protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

DNA-directed RNA polymerase II subunit RPB3, hRPB33, hsRPB3, RPB3, RPB31

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

33.8 kDa (298aa) confirmed by MALDI-TOF

POLR2C, DNA-directed RNA polymerase II subunit RPB3, hRPB33, hsRPB3, RPB3, RPB31, ATGP2724-10 µg, ATGP2724-20 µg, ATGP2724-50 µg, ATGP2724-100 µg, ATGP2724-250 µg, ATGP2724-500 µg, ATGP2724-1 mg, ATGP2724-10, ATGP2724-20, ATGP2724-50, ATGP2724-100, ATGP2724-250, ATGP2724-500, ATGP2724-1

Bertolotti, A. et al. (1998) Mol. Cell. Biol. 18:1489-1497.; ; Acker, J. et al. (1998) J. Biol. Chem. 272:16815-16821

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 0.1M NaCl

Epigenomics (Transcription & Translation)

NP_116558

P19387

Human

MPYANQP TVRITELTDE NVKFIIENTD LAVANSIRRV FIAEVPIIAI DWVQIDANSS VLHDEFIAHR LGLIPLISDD IVDKLQYSRD CTCEEFCPEC SVEFTLDVRC NEDQTRHVTS RDLISNSPRV IPVTSRNRDN DPNDYVEQDD ILIVKLRKGQ ELRLRAYAKK GFGKEHAKWN PTAGVAFEYD PDNALRHTVY PKPEEWPKSE YSELDEDESQ APYDPNGKPE RFYYNVESCG SLRPETIVLS ALSGLKKKLS DLQTQLSHEI QSDVLTIN