Recombinant humaan RNA polymerase III subeenheid RPC10/POLR3 K-eiwit

PORL3K is a small essential subunit of RNA polymerase III, the polymerase responsible for synthesizing transfer and small ribosomal RNAs in eukaryotes. The carboxy-terminal domain of this subunit shares a high degree of sequence similarity to the carboxy-terminal domain of an RNA polymerase II elongation factor. This similarity in sequence is supported by functional studies showing that this subunit is required for proper pausing and termination during transcription. Pseudogenes of this gene are found on chromosomes 13 and 17 Recombinant human PORL3K protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

DNA-directed RNA polymerase III subunit RPC10, C11, C11-RNP3, hRPC11, My010, RPC10, RPC11, RPC12.5

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

14.7 kDa (131aa)

POLR3K, DNA-directed RNA polymerase III subunit RPC10, C11, C11-RNP3, hRPC11, My010, RPC10, RPC11, RPC12.5, ATGP2759-10 µg, ATGP2759-20 µg, ATGP2759-50 µg, ATGP2759-100 µg, ATGP2759-250 µg, ATGP2759-500 µg, ATGP2759-1 mg, ATGP2759-10, ATGP2759-20, ATGP2759-50, ATGP2759-100, ATGP2759-250, ATGP2759-500, ATGP2759-1

Johnson, S.S., et al. (2007) Mol. Cell 26 (3), 367-379; ; De Gobbi, M., et al. (2006) Science 312 (5777), 1215-1217;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 50% glycerol, 2mM DTT, 2mM EDTA

Epigenomics (Transcription & Translation)

NP_057394.1

Q9Y2Y1

Human

MLLFCPG CGNGLIVEEG QRCHRFACNT CPYVHNITRK VTNRKYPKLK EVDDVLGGAA AWENVDSTAE SCPKCEHPRA YFMQLQTRSA DEPMTTFYKC CNAQCGHRWR D