Recombinant humaan RNA polymerase III subeenheid RPC6/POLR3F eiwit

POLR3F, also known as RPC39 and RPC6, belongs to the eukaryotic RPC34/RPC39 RNA polymerase subunit family. POLR3F is one of more than a dozen subunits forming eukaryotic RNA polymerase III (RNA Pol III), which transcribes 5S ribosomal RNA and tRNA genes. This protein has been shown to bind both TFIIIB90 and TBP, two subunits of RNA polymerase III transcription initiation factor IIIB (TFIIIB) . It is DNA-dependent RNA polymerase that catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Recombinant human POLR3F protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

DNA-directed RNA polymerase III subunit RPC6, RPC39, RPC6

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

38.1 kDa (339aa) confirmed by MALDI-TOF

POLR3F, DNA-directed RNA polymerase III subunit RPC6, RPC39, RPC6, ATGP1897-10 µg, ATGP1897-20 µg, ATGP1897-50 µg, ATGP1897-100 µg, ATGP1897-250 µg, ATGP1897-500 µg, ATGP1897-1 mg, ATGP1897-10, ATGP1897-20, ATGP1897-50, ATGP1897-100, ATGP1897-250, ATGP1897-500, ATGP1897-1

Chiu Y.-H., et al. (2009) Cell 138:576-591; ; Ablasser A., et al. (2009) Nat. Immunol. 10:1065-1072

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 30% glycerol, 1mM DTT

Epigenomics (Transcription & Translation)

NP_006457

Q9H1D9

Human

MGSSHHHHHH SSGLVPRGSH MGSMAEVKVK VQPPDADPVE IENRIIELCH QFPHGITDQV IQNEMPHIEA QQRAVAINRL LSMGQLDLLR SNTGLLYRIK DSQNAGKMKG SDNQEKLVYQ IIEDAGNKGI WSRDIRYKSN LPLTEINKIL KNLESKKLIK AVKSVAASKK KVYMLYNLQP DRSVTGGAWY SDQDFESEFV EVLNQQCFKF LQSKAETARE SKQNPMIQRN SSFASSHEVW KYICELGISK VELSMEDIET ILNTLIYDGK VEMTIIAAKE GTVGSVDGHM KLYRAVNPII PPTGLVRAPC GLCPVFDDCH EGGEISPSNC IYMTEWLEF