Recombinant humaan RNA polymerase III subeenheid RPC8/POLR3H-eiwit

POLR3H, also as known as A1665 and RPC8, belongs to the eukaryotic RPB7/RPC8 RNA polymerase subunit family. POLR3H is DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. It plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. POLR3H acts as nuclear and cytosolic DNA sensor involved in innate immune response. Recombinant human POLR3H protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

DNA-directed RNA polymerase III subunit RPC8, RPC22.9, RPC8

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

25.3 kDa (227aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

POLR3H, DNA-directed RNA polymerase III subunit RPC8, RPC22.9, RPC8, ATGP1755-10 µg, ATGP1755-20 µg, ATGP1755-50 µg, ATGP1755-100 µg, ATGP1755-250 µg, ATGP1755-500 µg, ATGP1755-1 mg, ATGP1755-10, ATGP1755-20, ATGP1755-50, ATGP1755-100, ATGP1755-250, ATGP1755-500, ATGP1755-1

Ablasser A., et al. (2009) Nat. Immunol. 10:1065-1072; ; Hu P., et al. (2002) Mol. Cell. Biol. 22:8044-8055

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol, 1mM DTT

Epigenomics (Transcription & Translation)

NP_612211

Q9Y535

Human

MGSSHHHHHH SSGLVPRGSH MGSMFVLVEM VDTVRIPPWQ FERKLNDSIA EELNKKLANK VVYNVGLCIC LFDITKLEDA YVFPGDGASH TKVHFRCVVF HPFLDEILIG KIKGCSPEGV HVSLGFFDDI LIPPESLQQP AKFDEAEQVW VWEYETEEGA HDLYMDTGEE IRFRVVDESF VDTSPTGPSS ADATTSSEEL PKKEAPYTLV GSISEPGLGL LSWWTSN