Recombinant humaan RNASE3-eiwit

RNASE3, also known as Ribonuclease RNase A family 3, located in the eosinophil primary matrix. It released during degranulation of eosinophils. This protein is related to inflammation and asthma because in these cases, there are increased levels of ECP in the body. It is a potent cytotoxic protein capable of killing cells of guinea pig tracheal epithelium, mammalian leukemia, epidermis carcinoma, and breast carcinoma, as well as non-mammalian cells such as parasites, bacteria, and viruses. Recombinant human RNASE3 protein, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Ribonuclease, RNase A family, 3 (eosinophil cationic protein), RNASE3, ECP, RAF1, RNS3

Baculovirus

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS-PAGE

16.6 kDa (142aa)

RNASE3, Ribonuclease, RNase A family, 3 (eosinophil cationic protein), RNASE3, ECP, RAF1, RNS3, ATGP3508-10 µg, ATGP3508-20 µg, ATGP3508-50 µg, ATGP3508-100 µg, ATGP3508-250 µg, ATGP3508-500 µg, ATGP3508-1 mg, ATGP3508-10, ATGP3508-20, ATGP3508-50, ATGP3508-100, ATGP3508-250, ATGP3508-500, ATGP3508-1

Kang I. et al., (2010) Eur Arch Otorhinolaryngol.267:391-395. ; ; Rubin J. et al., (2013) Mol Immunol. 55:372-380.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Enzymes & Proteases

AAH96060

P12724

Human

Ala

ADPRPPQFTR AQWFAIQHIS LNPPRCTIAM RAINNYRWRC KNQNTFLRTT FANVVNVCGN QSIRCPHNRT LNNCHRSRFR VPLLHCDLIN PGAQNISNCR YADRPGRRFY VVACDNRDPR DSPRYPVVPV HLDTTIHHHH HH