Recombinant humaan RND1-eiwit

RND1, also known as Rho 6, is Rho-related GTPases form a distinct branch of the Rho family, since they differ from other Rho proteins in size, charge, and biochemical properties. It appears to be constitutively in the activated GTP-bound form and acts as negative regulators of actin assembly and of cell adhesion. It contributes to regulating the organization of the actin cytoskeleton in response to extracellular growth factors. Recombinant human RND1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Rho-related GTP-binding protein Rho6, ARHS, FLJ42294, RHO6, RHO

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

24.5 kDa (220aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

RND1, Rho-related GTP-binding protein Rho6, ARHS, FLJ42294, RHO6, RHO, ATGP1148-10 µg, ATGP1148-20 µg, ATGP1148-50 µg, ATGP1148-100 µg, ATGP1148-250 µg, ATGP1148-500 µg, ATGP1148-1 mg, ATGP1148-10, ATGP1148-20, ATGP1148-50, ATGP1148-100, ATGP1148-250, ATGP1148-500, ATGP1148-1

Nobes C.D. et al. (1998) J. Cell Biol. 141: 187-197.; ; Garrett M.D. et al. (1989) J. Biol. Chem. 264: 10-13.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 30% glycerol, 0.1M NaCl,1mM DTT

Signal Transduction

NP_055285.1

Q92730

Human

MKERRAPQPV VARCKLVLVG DVQCGKTAML QVLAKDCYPE TYVPTVFENY TACLETEEQR VELSLWDTSG SPYYDNVRPL CYSDSDAVLL CFDISRPETV DSALKKWRTE ILDYCPSTRV LLIGCKTDLR TDLSTLMELS HQKQAPISYE QGCAIAKQLG AEIYLEGSAF TSEKSIHSIF RTASMLCLNK PSPLPQKSPV