Recombinant humaan RND3-eiwit

Rho-related GTP-binding protein RhoE, also known as RND3, is a small GTPase of the Ras superfamily that regulate the organization of the actin cytoskeleton in response to extracellular growth factors. RND3 activity leads to a decrease in stress fibers and increased cell migration, at least in part through regulation of the Rho-dependent kinase ROCK1. Also, RND3 has been implicated in inhibition of DNA-damage induced apoptosis and cell cycle arrest. Recombinant human RND3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Rho-related GTP-binding protein RhoE, ARHE, memB, Rho8, RhoE, Rho-related GTP-binding protein RhoE

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

29.2 kDa (261aa) confirmed by MALDI-TOF

RND3, Rho-related GTP-binding protein RhoE, ARHE, memB, Rho8, RhoE, Rho-related GTP-binding protein RhoE, ATGP0651-10 µg, ATGP0651-20 µg, ATGP0651-50 µg, ATGP0651-100 µg, ATGP0651-250 µg, ATGP0651-500 µg, ATGP0651-1 mg, ATGP0651-10, ATGP0651-20, ATGP0651-50, ATGP0651-100, ATGP0651-250, ATGP0651-500, ATGP0651-1

Riento K., et al. (2005) Mol Cell Biol. 23:4219-29.; ; Boswell S A., et al. (2007) J Biol Chem. 282:4850-8;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 40% glycerol, 1mM EDTA, 0.1M NaCl

Cancer

NP_005159

P61587

Human

MKERRASQKL SSKSIMDPNQ NVKCKIVVVG DSQCGKTALL HVFAKDCFPE NYVPTVFENY TASFEIDTQR IELSLWDTSG SPYYDNVRPL SYPDSDAVLI CFDISRPETL DSVLKKWKGE IQEFCPNTKM LLVGCKSDLR TDVSTLVELS NHRQTPVSYD QGANMAKQIG AATYIECSAL QSENSVRDIF HVATLACVNK TNKNVKRNKS QRATKRISHM PSRPELSAVA TDLRKDKAKS C