Recombinant humaan RNF181 eiwit

E3 ubiquitin-protein ligase RNF181, also known as RNF181, accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Recombinant human RNF181 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

E3 ubiquitin-protein ligase RNF181, HSPC238

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

20.3 kDa (176aa) confirmed by MALDI-TOF

RNF181, E3 ubiquitin-protein ligase RNF181, HSPC238, ATGP1717-10 µg, ATGP1717-20 µg, ATGP1717-50 µg, ATGP1717-100 µg, ATGP1717-250 µg, ATGP1717-500 µg, ATGP1717-1 mg, ATGP1717-10, ATGP1717-20, ATGP1717-50, ATGP1717-100, ATGP1717-250, ATGP1717-500, ATGP1717-1

Brophy T.M., et al. (2008) Biochem. Biophys. Res. Commun. 369:1088-1093; ; Gauci S., et al. (2009) Anal. Chem. 81:4493-4501

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Ubiquitination

NP_057578

Q9P0P0

Human

MGSSHHHHHH SSGLVPRGSH MGSMASYFDE HDCEPSDPEQ ETRTNMLLEL ARSLFNRMDF EDLGLVVDWD HHLPPPAAKT VVENLPRTVI RGSQAELKCP VCLLEFEEEE TAIEMPCHHL FHSSCILPWL SKTNSCPLCR YELPTDDDTY EEHRRDKARK QQQQHRLENL HGAMYT