Recombinant humaan RNF34-eiwit

RNF34contains a RINF finger, a motif known to be involved in protein-protein and protein-DNA interactions. This protein interacts with DNAJA3/hTid-1, which is a DnaJ protein reported to function as a modulator of apoptosis. Overexpression of this gene in Hela cells was shown to confer the resistance to TNF-alpha induced apoptosis, suggesting an anti-apoptotic function of this protein. This protein can be cleaved by caspase-3 during the induction of apoptosis. This protein also targets p53 and phospho-p53 for degradation. Alternatively splicing results in multiple transcript variants encoding distinct isoforms. Recombinant human RNF34 protein, fused to His-tag at N-terminus, was expressed in E. coli.

E3 ubiquitin-protein ligase RNF34 isoform 1, CARP-1, CARP1, Caspase regulator CARP1, Caspases-8 and-10-associated RING finger protein 1, E3 ubiquitin-protein ligase, ring finger protein 34, FLJ21786, RIF, RIFF, E3 ubiquitin protein ligase RNF34 isoform 1

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

44.2 kDa (396aa) confirmed by MALDI-TOF

RNF34, E3 ubiquitin-protein ligase RNF34 isoform 1, CARP-1, CARP1, Caspase regulator CARP1, Caspases-8 and-10-associated RING finger protein 1, E3 ubiquitin-protein ligase, ring finger protein 34, FLJ21786, RIF, RIFF, E3 ubiquitin protein ligase RNF34 isoform 1, ATGP2419-10 µg, ATGP2419-20 µg, ATGP2419-50 µg, ATGP2419-100 µg, ATGP2419-250 µg, ATGP2419-500 µg, ATGP2419-1 mg, ATGP2419-10, ATGP2419-20, ATGP2419-50, ATGP2419-100, ATGP2419-250, ATGP2419-500, ATGP2419-1

Wei P. et al. (2012) Mol Cell Biol. 32:266-275.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol 0.1M NaCl, 1mM DTT

Ubiquitination

NP_919247

Q969K3

Human

MGSSHHHHHH SSGLVPRGSH MGSMRKAGAT SMWASCCGLL NEVMGTGAVR GQQSAFAGAT GPFRFTPNPE FSTYPPAATE GPNIVCKACG LSFSVFRKKH VCCDCKKDFC SVCSVLQENL RRCSTCHLLQ ETAFQRPQLM RLKVKDLRQY LILRNIPIDT CREKEDLVDL VLCHHGLGSE DDMDTSSLNS SRSQTSSFFT RSFFSNYTAP SATMSSFQGE LMDGDQTSRS GVPAQVQSEI TSANTEDDDD DDDEDDDDEE ENAEDRNPGL SKERVRASLS DLSSLDDVEG MSVRQLKEIL ARNFVNYSGC CEKWELVEKV NRLYKENEEN QKSYGERLQL QDEEDDSLCR ICMDAVIDCV LLECGHMVTC TKCGKRMSEC PICRQYVVRA VHVFKS