Recombinant humaan RNF4-eiwit

E3 ubiquitin ligase RNF4, also known as RNF4, contains a RING finger motif and acts as a transcription regulator. This protein has been shown to interact with, and inhibit the activity of, TRPS1, a transcription suppressor of GATA-mediated transcription. Transcription repressor ZNF278/PATZ is found to interact with this protein, and thus reduce the enhancement of androgen receptor-dependent transcription mediated by this protein. Recombinant human RNF4 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

E3 ubiquitin ligase RNF4, RES4-26, SLX5, SNuRF

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

23.7 kDa (213aa) confirmed by MALDI-TOF

RNF4, E3 ubiquitin ligase RNF4, RES4-26, SLX5, SNuRF, ATGP1761-10 µg, ATGP1761-20 µg, ATGP1761-50 µg, ATGP1761-100 µg, ATGP1761-250 µg, ATGP1761-500 µg, ATGP1761-1 mg, ATGP1761-10, ATGP1761-20, ATGP1761-50, ATGP1761-100, ATGP1761-250, ATGP1761-500, ATGP1761-1

Kaiser F.J., et al. (2003) J. Biol. Chem. 278:38780-38785; ; Guo B., et al. (2009) Mol. Cell. Biol. 29:3204-3218

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 30% glycerol, 1mM DTT

Ubiquitination

NP_001171938.1

P78317

Human

MSTRKRR GGAINSRQAQ KRTREATSTP EISLEAEPIE LVETAGDEIV DLTCESLEPV VVDLTHNDSV VIVDERRRPR RNARRLPQDH ADSCVVSSDD EELSRDRDVY VTTHTPRNAR DEGATGLRPS GTVSCPICMD GYSEIVQNGR LIVSTECGHV FCSQCLRDSL KNANTCPTCR KKINHKRYHP IYI