Recombinant humaan RNF7-eiwit

RNF7 is a highly conserved ring finger protein. It is an essential subunit of SKP1-cullin/CDC53-F box protein ubiquitin ligases, which are a part of the protein degradation machinery important for cell cycle progression and signal transduction. This protein interacts with, and is a substrate of, casein kinase II (CSNK2A1/CKII) . The phosphorylation of this protein by CSNK2A1 has been shown to promote the degradation of IkappaBalpha (CHuK/IKK-alpha/IKBKA) and p27Kip1 (CDKN1B) . Alternatively spliced transcript variants encoding distinct isoforms have been reported. Recombinant human RNF7 protein, fused to His-tag at N-terminus, was expressed in E. coli.

RING-box protein 2 isoform 1, Ring finger protein 7, CKBBP1, ROC2, SAG

E.coli

Human

His-Tag

SDS-PAGE, Denatured

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

15.1 kDa (136aa)

RNF7, RING-box protein 2 isoform 1, Ring finger protein 7, CKBBP1, ROC2, SAG, ATGP2286-10 µg, ATGP2286-20 µg, ATGP2286-50 µg, ATGP2286-100 µg, ATGP2286-250 µg, ATGP2286-500 µg, ATGP2286-1 mg, ATGP2286-10, ATGP2286-20, ATGP2286-50, ATGP2286-100, ATGP2286-250, ATGP2286-500, ATGP2286-1

Swaroop M., et al. (2000) . Oncogene 19:2855-2866

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.4M urea

Metabolism

NP_055060

Q9UBF6

Human

MADVEDG EETCALASHS GSSGSKSGGD KMFSLKKWNA VAMWSWDVEC DTCAICRVQV MDACLRCQAE NKQEDCVVVW GECNHSFHNC CMSLWVKQNN RCPLCQQDWV VQRIGK