Recombinant humaan RNGTT-eiwit

RNGTT is a bifunctional mRNA-capping enzyme exhibiting RNA 5'-triphosphatase activity in the N-terminal part and mRNA guanylyltransferase activity in the C-terminal part. It catalyzes the first two steps of cap formation: by removing the gamma-phosphate from the 5'-triphosphate end of nascent mRNA to yield a diphosphate end, and by transferring the gmp moiety of GTP to the 5'-diphosphate terminus. Recombinant human RNGTT protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

MRNA-capping enzyme, RNA guanylyltransferase and 5'-phosphatase, CAP1A, hCAP, HCE, HCE1

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

70.9 kDa (620aa)

RNGTT, mRNA-capping enzyme, RNA guanylyltransferase and 5'-phosphatase, CAP1A, hCAP, HCE, HCE1, ATGP2805-10 µg, ATGP2805-20 µg, ATGP2805-50 µg, ATGP2805-100 µg, ATGP2805-250 µg, ATGP2805-500 µg, ATGP2805-1 mg, ATGP2805-10, ATGP2805-20, ATGP2805-50, ATGP2805-100, ATGP2805-250, ATGP2805-500, ATGP2805-1

Yue Z., Maldonado E et al. (1997) Proc. Natl. Acad. Sci. u.S.A. 94:12898-12903.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 7.5) containing 0.2M NaCl, 40% glycerol, 2mM DTT, 0.1mM PMSF

Epigenomics (Transcription & Translation)

NP_003791

O60942

Human

MAHNKIP PRWLNCPRRG QPVAGRFLPL KTMLGPRYDS QVAEENRFHP SMLSNYLKSL KVKMGLLVDL TNTSRFYDRN DIEKEGIKYI KLQCKGHGEC PTTENTETFI RLCERFNERN PPELIGVHCT HGFNRTGFLI CAFLVEKMDW SIEAAVATFA QARPPGIYKG DYLKELFRRY GDIEEAPPPP LLPDWCFEDD EDEDEDEDGK KESEPGSSAS FGKRRKERLK LGAIFLEGVT VKGVTQVTTQ PKLGEVQQKC HQFCGWEGSG FPGAQPVSMD KQNIKLLDLK PYKVSWKADG TRYMMLIDGT NEVFMIDRDN SVFHVSNLEF PFRKDLRMHL SNTLLDGEMI IDRVNGQAVP RYLIYDIIKF NSQPVGDCDF NVRLQCIERE IISPRHEKMK TGLIDKTQEP FSVRNKPFFD ICTSRKLLEG NFAKEVSHEM DGLIFQPTGK YKPGRCDDIL KWKPPSLNSV DFRLKITRMG GEGLLPQNVG LLYVGGYERP FAQIKVTKEL KQYDNKIIEC KFENNSWVFM RQRTDKSFPN AYNTAMAVCN SISNPVTKEM LFEFIDRCTA ASQGQKRKHH LDPDTELMPP PPPKRPRPLT