Recombinant humaan RNMT-eiwit

RNMT (mRNA cap guanine-N7 methyltransferase), also known as MET, RG7MT1 or hCMT1c, is a widely expressed nuclear protein that belongs to the mRNA cap methyltransferase family. Cap-dependent mRNA translation requires the methylation of the mRNA guanosine cap by RNMT. RNMT catalyzes the transfer of a methyl group from AdoMet (S-adenosylmethionine) to the GpppN end of the growing mRNA at the N-7 position, thereby producing AdoHyc (S-adenosylhomocysteine) and m7GpppN terminated RNA. Recombinant human RNMT protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

MRNA cap guanine-N7 methyltransferase, MET, RG7MT1, DKFZp686H1252, EC 2.1.1.56, hcm1p, hCMT1, HCMT1a, hCMT1c, hMet, hypothetical protein KIAA0396, KIAA0398, MET, mRNA (guanine 7) methyltransferase, mRNA (guanine N (7) ) methyltransferase, mRNA cap methyltransferase, OTTHuMP00000162509, RNA guanine 7 methyltransferase, RNA guanine-7-methyltransferase, RNMT

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

57 kDa (496aa)

RNMT, mRNA cap guanine-N7 methyltransferase, MET, RG7MT1, DKFZp686H1252, EC 2.1.1.56, hcm1p, hCMT1, HCMT1a, hCMT1c, hMet, hypothetical protein KIAA0396, KIAA0398, MET, mRNA (guanine 7) methyltransferase, mRNA (guanine N (7) ) methyltransferase, mRNA cap methyltransferase, OTTHuMP00000162509, RNA guanine 7 methyltransferase, RNA guanine-7-methyltransferase, RNMT, ATGP1068-10 µg, ATGP1068-20 µg, ATGP1068-50 µg, ATGP1068-100 µg, ATGP1068-250 µg, ATGP1068-500 µg, ATGP1068-1 mg, ATGP1068-10, ATGP1068-20, ATGP1068-50, ATGP1068-100, ATGP1068-250, ATGP1068-500, ATGP1068-1

Cowling VH, et al. (2010) Genes Cancer. 1 (6) :576-579.; ; Cowling VH, et al. (2010) Oncogene. 29 (6) :930-6.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 20% glycerol, 100 mM NaCl

Epigenomics (Transcription & Translation)

NP_003790.1

O43148

Human

MANSAKAEEY EKMSLEQAKA SVNSETESSF NINENTTASG TGLSEKTSVC RQVDIARKRK EFEDDLVKES SSCGKDTPSK KRKLDPEIVP EEKDCGDAEG NSKKRKRETE DVPKDKSSTG DGTQNKRKIA LEDVPEKQKN LEEGHSSTVA AHYNELQEVG LEKRSQSRIF YLRNFNNWMK SVLIGEFLEK VRQKKKRDIT VLDLGCGKGG DLLKWKKGRI NKLVCTDIAD VSVKQCQQRY EDMKNRRDSE YIFSAEFITA DSSKELLIDK FRDPQMCFDI CSCQFVCHYS FESYEQADMM LRNACERLSP GGYFIGTTPN SFELIRRLEA SETESFGNEI YTVKFQKKGD YPLFGCKYDF NLEGVVDVPE FLVYFPLLNE MAKKYNMKLV YKKTFLEFYE EKIKNNENKM LLKRMQALEP YPANESSKLV SEKVDDYEHA AKYMKNSQVR LPLGTLSKSE WEATSIYLVF AFEKQQ