Recombinant humaan ROBLD3/LAMTOR2-eiwit

ROBLD3, also known as MAPBPIP, belongs to the GAMAD family. It is an adapter protein that enhances the efficiency of the MAP kinase cascade and facilitates the activation of MAPK2. ROBLD3 compels the recruitment of MP1 to late endosomes where they form a very stable heterodimeric complex required for ERK activation on endosomes. Recombinant human ROBLD3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

ENDAP, Endosomal adaptor protein p14, Late endosomal/lysosomal adaptor and MAPK and MTOR activator 2, MAPBP-interacting protein, MAPBPIP, MAPKSP1 adaptor protein, MAPKSP1AP, Mitogen activated protein binding protein interacting protein, p14, Ragulator complex protein LAMTOR2, Ragulator2, Roadblock domain containing 3

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by BCA)

> 95% by SDS-PAGE

16 kDa (149aa) confirmed by MALDI-TOF

ENDAP, Endosomal adaptor protein p14, HSPC003, LAMTOR2, Late endosomal/lysosomal adaptor and MAPK and MTOR activator 2, MAPBP-interacting protein, MAPBPIP, MAPKSP1 adaptor protein, MAPKSP1AP, Mitogen activated protein binding protein interacting protein, Mitogen-activated protein-binding protein-interacting protein, p14, Ragulator complex protein LAMTOR2, Ragulator2, Roadblock domain containing 3, ROBLD3, RP11-336K24.9, ATGP0834-10 µg, ATGP0834-20 µg, ATGP0834-50 µg, ATGP0834-100 µg, ATGP0834-250 µg, ATGP0834-500 µg, ATGP0834-1 mg, ATGP0834-010, ATGP0834-020, ATGP0834-050, ATGP0834-100, ATGP0834-250, ATGP0834-500, ATGP0834-01M

Sancak Y., et al. (2010) Cell. 141 (2) :290-303.; ; Waters S., et al. (2009) FEBS Lett. 583 (12) :1846-52.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M Nacl, 10% glycerol

Signal Transduction

NP_054736.1

Q9Y2Q5

Human

MLRPKA LTQVLSQANT GGVQSTLLLN NEGSLLAYSG YGDTDARVTA AIASNIWAAY DRNGNQAFNE DNLKFILMDC MEGRVAITRV ANLLLCMYAK ETVGFGMLKA KAQALVQYLE EPLTQVAAS