Recombinant humaan ROBLD3/LAMTOR2-eiwit

LAMTOR2 is highly conserved with a mouse protein associated with the cytoplasmic face of late endosomes and lysosomes. The mouse protein interacts with MAPK scaffold protein 1, a component of the mitogen-activated protein kinase pathway. In humans, a mutation in this gene has been associated with a primary immunodeficiency syndrome, and suggests a role for this protein in endosomal biogenesis. Recombinant human LAMTOR2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

ENDAP, Endosomal adaptor protein p14, Late endosomal/lysosomal adaptor and MAPK and MTOR activator 2, MAPBP-interacting protein, MAPBPIP, MAPKSP1 adaptor protein, MAPKSP1AP, Mitogen activated protein binding protein interacting protein, p14, Ragulator complex protein LAMTOR2, Ragulator2, Roadblock domain containing 3

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

15.9 kDa (148aa) confirmed by MALDI-TOF

ENDAP, Endosomal adaptor protein p14, HSPC003, LAMTOR2, Late endosomal/lysosomal adaptor and MAPK and MTOR activator 2, MAPBP-interacting protein, MAPBPIP, MAPKSP1 adaptor protein, MAPKSP1AP, Mitogen activated protein binding protein interacting protein, Mitogen-activated protein-binding protein-interacting protein, p14, Ragulator complex protein LAMTOR2, Ragulator2, Roadblock domain containing 3, ROBLD3, RP11-336K24.9, ATGP2067-10 µg, ATGP2067-20 µg, ATGP2067-50 µg, ATGP2067-100 µg, ATGP2067-250 µg, ATGP2067-500 µg, ATGP2067-1 mg, ATGP2067-010, ATGP2067-020, ATGP2067-050, ATGP2067-100, ATGP2067-250, ATGP2067-500, ATGP2067-01M

Bohn G., et al. (2007) Nat. Med. 13:38-45; ; Sancak Y., et al. (2010) Cell. 141:290-303;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Signal Transduction

NP_054736

Q9Y2Q5

Human

MGSSHHHHHH SSGLVPRGSH MGSMLRPKAL TQVLSQANTG GVQSTLLLNN EGSLLAYSGY GDTDARVTAA IASNIWAAYD RNGNQAFNED NLKFILMDCM EGRVAITRVA NLLLCMYAKE TVGFGMLKAK AQALVQYLEE PLTQVAAS