Recombinant humaan RPA2-eiwit

Replication Protein A (RPA) is a single stranded DNA binding protein. Human RPA is a heterotrimeric protein containing subunits of 14, 32 and 70kDa. This protein complex is highly conserved in eukaryotes and is essential in DNA replication, homologous recombination and nucleotide excision repair. The C-terminus of RPA2 can specfically intereact with the DNA repair enzyme uNG2 and repair factors XPA and Rad52, each of which functions in a different repair pathway. In addition, this protein binds specifically to the SH2 domain of Stat3 in vivo, and overexpression of RPA2 corresponds to the augmented growth factor-stimulated tyrosine phosphorylation and transcription activities of Stat3. Recombinant human RPA2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Replication protein A 32 kDa subunit, Replication protein A 32 kDa subunit, REPA2, RPA32, RPA32, RP-A p32, RP-A p34

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

31.7 kDa (293aa) confirmed by MALDI-TOF

RPA2, ATGP0792-10 µg, ATGP0792-20 µg, ATGP0792-50 µg, ATGP0792-100 µg, ATGP0792-250 µg, ATGP0792-500 µg, ATGP0792-1 mg, ATGP0792-10, ATGP0792-20, ATGP0792-50, ATGP0792-100, ATGP0792-250, ATGP0792-500, ATGP0792-1

Wang M., et al. (2000) Biochemistry. 39 (21) :6433-9. ; ; Mer G., et al. (2000) Cell. 103 (3) :449-56.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl, 2mM DTT

Epigenomics (Transcription & Translation)

NP_002937

P15927

Human

MWNSGFE SYGSSSYGGA GGYTQSPGGF GSPAPSQAEK KSRARAQHIV PCTISQLLSA TLVDEVFRIG NVEISQVTIV GIIRHAEKAP TNIVYKIDDM TAAPMDVRQW VDTDDTSSEN TVVPPETYVK VAGHLRSFQN KKSLVAFKIM PLEDMNEFTT HILEVINAHM VLSKANSQPS AGRAPISNPG MSEAGNFGGN SFMPANGLTV AQNQVLNLIK ACPRPEGLNF QDLKNQLKHM SVSSIKQAVD FLSNEGHIYS TVDDDHFKST DAE