Recombinant humaan RPE-eiwit

RPE (ribulose-phosphate 3-epimerase) belongs to the ribulose-phosphate 3-epimerase family. In enzymology, a ribulose-phosphate 3-epimerase is the enzyme that converts D-ribulose 5-phosphate into D-xylulose 5-phosphate in Calvin's reductive pentose phosphate cycle. Hence, this enzyme has one substrate, D-ribulose 5-phosphate, and one product, D-xylulose 5-phosphate. This enzyme participates in 3 metabolic pathways: pentose phosphate pathway, pentose and glucuronate interconversions, and carbon fixation. Recombinant human RPE protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Ribulose-5-phosphate-3-epimerase, RPE2-1

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

27.5 kDa (252aa) confirmed by MALDI-TOF

RPE, ribulose-5-phosphate-3-epimerase, RPE2-1, ATGP1439-10 µg, ATGP1439-20 µg, ATGP1439-50 µg, ATGP1439-100 µg, ATGP1439-250 µg, ATGP1439-500 µg, ATGP1439-1 mg, ATGP1439-10, ATGP1439-20, ATGP1439-50, ATGP1439-100, ATGP1439-250, ATGP1439-500, ATGP1439-1

Liang W., et al. (2011) FASEB J. 25:497-504; ; Akana, J., et al. (2006) Biochemistry 45: 2493-2503.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 30% glycerol, 200 mM NaCl

Metabolism

NP_954699

Q96AT9

Human

MASGCK IGPSILNSDL ANLGAECLRM LDSGADYLHL DVMDGHFVPN ITFGHPVVES LRKQLGQDPF FDMHMMVSKP EQWVKPMAVA GANQYTFHLE ATENPGALIK DIRENGMKVG LAIKPGTSVE YLAPWANQID MALVMTVEPG FGGQKFMEDM MPKVHWLRTQ FPSLDIEVDG GVGPDTVHKC AEAGANMIVS GSAIMRSEDP RSVINLLRNV CSEAAQKRSL DR