Recombinant humaan RPIA-eiwit

RPIA (Ribose-5-phosphate isomerase) belongs to the ribose 5-phosphate isomerase family. RPIA is an enzyme that catalyzes the conversion between ribose-5-phosphate (R5P) and ribulose-5-phosphate (Ru5P) . RPI exists as two distinct proteins forms, termed RPIA and RPIB. RPIA plays an essential role in the metabolism of plants and animals, as it is involved in the Calvin cycle which takes place in plants, and the pentose phosphate pathway which takes place in plants as well as animals. Recombinant human RPIA protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Ribose 5-phosphate isomerase, Phosphoriboisomerase, RPI

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

35.4 kDa (331aa) confirmed by MALDI-TOF

RPIA, Ribose 5-phosphate isomerase, Phosphoriboisomerase, RPI, ATGP1356-10 µg, ATGP1356-20 µg, ATGP1356-50 µg, ATGP1356-100 µg, ATGP1356-250 µg, ATGP1356-500 µg, ATGP1356-1 mg, ATGP1356-10, ATGP1356-20, ATGP1356-50, ATGP1356-100, ATGP1356-250, ATGP1356-500, ATGP1356-1

Zhang, R., et al. (2003) Structure 11 (1) : 31.; ; Huck J.H.J., et al. (2004) Am. J. Hum. Genet. 74:745-751

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 40% glycerol, 200 mM NaCl, 2mM EDTA, 0,2mM PMSF

Metabolism

NP_653164.2

P49247

Human

MQRPGPFSTL YGRVLAPLPG RAGGAASGGG GNSWDLPGSH VRLPGRAQSG TRGGAGNTST SCGDSNSICP APSTMSKAEE AKKLAGRAAV ENHVRNNQVL GIGSGSTIVH AVQRIAERVK QENLNLVCIP TSFQARQLIL QYGLTLSDLD RHPEIDLAID GADEVDADLN LIKGGGGCLT QEKIVAGYAS RFIVIADFRK DSKNLGDQWH KGIPIEVIPM AYVPVSRAVS QKFGGVVELR MAVNKAGPVV TDNGNFILDW KFDRVHKWSE VNTAIKMIPG VVDTGLFINM AERVYFGMQD GSVNMREKPF C