Recombinant humaan RPL11-eiwit

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. RPL11 is a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L5P family of ribosomal proteins. It is located in the cytoplasm. The protein probably associates with the 5S rRNA. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Recombinant human RPL11 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Ribosomal protein L11, DBA7, GIG34, L11

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

22.6 kDa (201aa) confirmed by MALDI-TOF

RPL11, Ribosomal protein L11, DBA7, GIG34, L11, ATGP2220-10 µg, ATGP2220-20 µg, ATGP2220-50 µg, ATGP2220-100 µg, ATGP2220-250 µg, ATGP2220-500 µg, ATGP2220-1 mg, ATGP2220-10, ATGP2220-20, ATGP2220-50, ATGP2220-100, ATGP2220-250, ATGP2220-500, ATGP2220-1

Mahata, B., et al. (2012) Oncogene 31 (25), 3060-3071; ; Dai, M.S, et al. (2012) J. Biol. Chem. 287 (21), 17120-17129

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 50% glycerol, 1mM DTT, 2mM EDTA, 250 mM Imidazole

Epigenomics (Transcription & Translation)

NP_000966

P62913

Human

MAQDQGE KENPMRELRI RKLCLNICVG ESGDRLTRAA KVLEQLTGQT PVFSKARYTV RSFGIRRNEK IAVHCTVRGA KAEEILEKGL KVREYELRKN NFSDTGNFGF GIQEHIDLGI KYDPSIGIYG LDFYVVLGRP GFSIADKKRR TGCIGAKHRI SKEEAMRWFQ QKYDGIILPG K