Recombinant humaan RPL12-eiwit

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. RPL12 is a ribosomal protein that is a component of the 60S subunit. This protein belongs to the L11P family of ribosomal proteins. It is located in the cytoplasm. RPL12 binds directly to the 26S rRNA. Recombinant human RPL12 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Ribosomal protein L12, L12

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

20.2 kDa (188aa) confirmed by MALDI-TOF

RPL12, Ribosomal protein L12, L12, ATGP1973-10 µg, ATGP1973-20 µg, ATGP1973-50 µg, ATGP1973-100 µg, ATGP1973-250 µg, ATGP1973-500 µg, ATGP1973-1 mg, ATGP1973-10, ATGP1973-20, ATGP1973-50, ATGP1973-100, ATGP1973-250, ATGP1973-500, ATGP1973-1

Rush J., et al. (2005) Nat. Biotechnol. 23:94-101; ; Daub H., et al. (2008) Mol. Cell. 31:438-448;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl,20% glycerol, 1mM DTT

Epigenomics (Transcription & Translation)

NP_000967

P30050

Human

MPPKFDP NEIKVVYLRC TGGEVGATSA LAPKIGPLGL SPKKVGDDIA KATGDWKGLR ITVKLTIQNR QAQIEVVPSA SALIIKALKE PPRDRKKQKN IKHSGNITFD EIVNIARQMR HRSLARELSG TIKEILGTAQ SVGCNVDGRH PHDIIDDINS GAVECPAS