Recombinant humaan RPL23A-eiwit

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. RPL23A is a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L23P family of ribosomal proteins. It is located in the cytoplasm. The protein may be one of the target molecules involved in mediating growth inhibition by interferon. In yeast, the corresponding protein binds to a specific site on the 26S rRNA. Recombinant human RPL23A protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Ribosomal protein L23a, L23A, MDA20

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 80% by SDS-PAGE

20.1 kDa (179aa) confirmed by MALDI-TOF

RPL23A, Ribosomal protein L23a, L23A, MDA20, ATGP2102-10 µg, ATGP2102-20 µg, ATGP2102-50 µg, ATGP2102-100 µg, ATGP2102-250 µg, ATGP2102-500 µg, ATGP2102-1 mg, ATGP2102-10, ATGP2102-20, ATGP2102-50, ATGP2102-100, ATGP2102-250, ATGP2102-500, ATGP2102-1

Choudhary C., et al. (2009) Science. 325:834-840; ; Wang B., et al. (2008) Anal. Chem. 80:9526-9533

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 50% glycerol, 2mM DTT, 250 mM imidazole

Epigenomics (Transcription & Translation)

NP_000975

P62750

Human

MGSSHHHHHH SSGLVPRGSH MGSMAPKAKK EAPAPPKAEA KAKALKAKKA VLKGVHSHKK KKIRTSPTFR RPKTLRLRRQ PKYPRKSAPR RNKLDHYAII KFPLTTESAM KKIEDNNTLV FIVDVKANKH QIKQAVKKLY DIDVAKVNTL IRPDGEKKAY VRLAPDYDAL DVANKIGII