Recombinant humaan RPL35A-eiwit

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. RPL35A is a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L35AE family of ribosomal proteins. It is located in the cytoplasm. The rat protein has been shown to bind to both initiator and elongator tRNAs, and thus, it is located at the P site, or P and A sites, of the ribosome. Although this gene was originally mapped to chromosome 18, it has been established that it is located at 3q29-qter. Transcript variants utilizing alternative transcription initiation sites and alternative polyA signals exist. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Recombinant human RPL35A protein, fused to His-tag at N-terminus, was expressed in E. coli.

60S ribosomal protein L35a

E.coli

Human

His-Tag

SDS-PAGE, Denatured

0.5 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

14.9 kDa (133aa)

RPL35A, 60S ribosomal protein L35a, ATGP2335-10 µg, ATGP2335-20 µg, ATGP2335-50 µg, ATGP2335-100 µg, ATGP2335-250 µg, ATGP2335-500 µg, ATGP2335-1 mg, ATGP2335-10, ATGP2335-20, ATGP2335-50, ATGP2335-100, ATGP2335-250, ATGP2335-500, ATGP2335-1

Lopez CD, Martinovsky G, et al. (2002) . Cancer Lett. 180 (2) :195-202.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol

Epigenomics (Transcription & Translation)

NP_000987

P18077

Human

MSGRLWS KAIFAGYKRG LRNQREHTAL LKIEGVYARD ETEFYLGKRC AYVYKAKNNT VTPGGKPNKT RVIWGKVTRA HGNSGMVRAK FRSNLPAKAI GHRIRVMLYP SRI