Recombinant humaan RPL8-eiwit

RPL8 (60S ribosomal protein L8) belongs to the ribosomal protein L2P family. Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. The protein belongs to the L2P family of ribosomal proteins. It is located in the cytoplasm and exists as a component of the 60S subunit where it is thought to play a role in aminoacyl-tRNA binding, specifically at the ribosomal subunit interface. Recombinant human RPL8 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

60S ribosomal protein L8, L8

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

30.1 kDa (277aa) confirmed by MALDI-TOF

RPL8, 60S ribosomal protein L8, L8, ATGP1442-10 µg, ATGP1442-20 µg, ATGP1442-50 µg, ATGP1442-100 µg, ATGP1442-250 µg, ATGP1442-500 µg, ATGP1442-1 mg, ATGP1442-10, ATGP1442-20, ATGP1442-50, ATGP1442-100, ATGP1442-250, ATGP1442-500, ATGP1442-1

Kenmochi, N., et al. (1998) Genome Res. 8: 509-523.; ; Hanes J, et al. (1994) Biochem Biophys Res Commun 197 (3) : 1223-8.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 50% glycerol, 300 mM NaCl, 2mM EDTA

Signal Transduction

NP_000964

P62917

Human

MGRVIRGQRK GAGSVFRAHV KHRKGAARLR AVDFAERHGY IKGIVKDIIH DPGRGAPLAK VVFRDPYRFK KRTELFIAAE GIHTGQFVYC GKKAQLNIGN VLPVGTMPEG TIVCCLEEKP GDRGKLARAS GNYATVISHN PETKKTRVKL PSGSKKVISS ANRAVVGVVA GGGRIDKPIL KAGRAYHKYK AKRNCWPRVR GVAMNPVEHP FGGGNHQHIG KPSTIRRDAP AGRKVGLIAA RRTGRLRGTK TVQEKEN