Recombinant humaan RPN2-eiwit

RPN2, also known as ribophorin 2, is a dolichyl-diphosphooligosaccharide protein glycosyltransferase subunit 2. This protein is part of an N-oligosaccharyl transferase complex that links high mannose oligosaccharides to asparagine residues found in the Asn-X-Ser/Thr consensus motif of nascent polypeptide chains. It is similar in sequence to the yeast oligosaccharyl transferase subunit SWP1. Recombinant human RPN2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Ribophorin II, SWP1, RPNII, RIBIIR, RPN-II

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

59.2 kDa (539aa)

RPN2, Ribophorin II, SWP1, RPNII, RIBIIR, RPN-II, ATGP1900-10 µg, ATGP1900-20 µg, ATGP1900-50 µg, ATGP1900-100 µg, ATGP1900-250 µg, ATGP1900-500 µg, ATGP1900-1 mg, ATGP1900-10, ATGP1900-20, ATGP1900-50, ATGP1900-100, ATGP1900-250, ATGP1900-500, ATGP1900-1

Skarnes WC. et al. (2011) Nature. 474:337-342.; ; Stoffel M. et al. (1992) Hum Mol Genet. 1:656.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol 0.1M NaCl

Enzymes & Proteases

NP_002942

P04844

Human

LTPTHYLTK HDVERLKASL DRPFTNLESA FYSIVGLSSL GAQVPDAKKA CTYIRSNLDP SNVDSLFYAA QASQALSGCE ISISNETKDL LLAAVSEDSS VTQIYHAVAA LSGFGLPLAS QEALSALTAR LSKEETVLAT VQALQTASHL SQQADLRSIV EEIEDLVARL DELGGVYLQF EEGLETTALF VAATYKLMDH VGTEPSIKED QVIQLMNAIF SKKNFESLSE AFSVASAAAV LSHNRYHVPV VVVPEGSASD THEQAILRLQ VTNVLSQPLT QATVKLEHAK SVASRATVLQ KTSFTPVGDV FELNFMNVKF SSGYYDFLVE VEGDNRYIAN TVELRVKIST EVGITNVDLS TVDKDQSIAP KTTRVTYPAK AKGTFIADSH QNFALFFQLV DVNTGAELTP HQTFVRLHNQ KTGQEVVFVA EPDNKNVYKF ELDTSERKIE FDSASGTYTL YLIIGDATLK NPILWNVADV VIKFPEEEAP STVLSQNLFT PKQEIQHLFR EPEKRPPTV