Recombinant humaan RPP30-eiwit

RPP30 (Ribonuclease P protein subunit p30) belongs to the eukaryotic/archaeal RNase P protein component 3 family. RPP30 is component of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends. Ribonuclease P (PNase P) is small nuclear ribonucleoproteins (snRNPs) that act on RNA substrates in vitro. Also, RNase P accumulates in the nucleolus, have a similar RNA component and also have several protein subunits in common. Recombinant human RPP30 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Ribonuclease P protein subunit p30, RNASEP2, TSG15

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

31.8 kDa (291aa) confirmed by MALDI-TOF

RPP30, Ribonuclease P protein subunit p30, RNASEP2, TSG15, ATGP0821-10 µg, ATGP0821-20 µg, ATGP0821-50 µg, ATGP0821-100 µg, ATGP0821-250 µg, ATGP0821-500 µg, ATGP0821-1 mg, ATGP0821-10, ATGP0821-20, ATGP0821-50, ATGP0821-100, ATGP0821-250, ATGP0821-500, ATGP0821-1

Kurz, J.C., et al. (2000) Curr. Opin. Chem. Biol. 4: 553-558.; ; van Eenennaam, H., et al. (2001) Mol. Biol. Cell. 12: 3680-3689.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 5mM DTT, 200 mM NaCl, 1mM EDTA

Metabolism

NP_006404

P78346

Human

MAVFADL DLRAGSDLKA LRGLVETAAH LGYSVVAINH IVDFKEKKQE IEKPVAVSEL FTTLPIVQGK SRPIKILTRL TIIVSDPSHC NVLRATSSRA RLYDVVAVFP KTEKLFHIAC THLDVDLVCI TVTEKLPFYF KRPPINVAID RGLAFELVYS PAIKDSTMRR YTISSALNLM QICKGKNVII SSAAERPLEI RGPYDVANLG LLFGLSESDA KAAVSTNCRA ALLHGETRKT AFGIISTVKK PRPSEGDEDC LPASKKAKCE G