Recombinant humaan RPRD1A-eiwit

RPRD1A is upregulated in cells overexpressing cyclin-dependent kinase inhibitor p15 (INK4b) and may have a role in cell cycle regulation Recombinant human RPRD1A protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Regulation of nuclear pre-mRNA domain-containing protein 1A, Regulation of nuclear pre-mRNA domain containing 1A, HsT3101, P15RS

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

38.4 kDa (337aa) confirmed by MALDI-TOF

RPRD1A, Regulation of nuclear pre-mRNA domain-containing protein 1A, Regulation of nuclear pre-mRNA domain containing 1A, HsT3101, P15RS, ATGP2648-10 µg, ATGP2648-20 µg, ATGP2648-50 µg, ATGP2648-100 µg, ATGP2648-250 µg, ATGP2648-500 µg, ATGP2648-1 mg, ATGP2648-10, ATGP2648-20, ATGP2648-50, ATGP2648-100, ATGP2648-250, ATGP2648-500, ATGP2648-1

Liu J., Liu H., Zhang X., et al. (2002) Biochem. Biophys. Res. Commun. 299:880-885; ; Ni Z., Olsen J.B., et al. (2011) Transcription 2:237-242;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT

Cell Cycle

NP_060640

Q96P16

Human

MGSSHHHHHH SSGLVPRGSH MGSEFMSAFS EAALEKKLSE LSNSQQSVQT LSLWLIHHRK HSRPIVTVWE RELRKAKPNR KLTFLYLAND VIQNSKRKGP EFTKDFAPVI VEAFKHVSSE TDESCKKHLG RVLSIWEERS VYENDVLEQL KQALYGDKKP RKRTYEQIKV DENENCSSLG SPSEPPQTLD LVRALQDLEN AASGDAAVHQ RIASLPVEVQ EVSLLDKITD KESGERLSKM VEDACMLLAD YNGRLAAEID DRKQLTRMLA DFLRCQKEAL AEKEHKLEEY KRKLARVSLV RKELRSRIQS LPDLSRLPNV TGSHMHLPFA GDIYSED