Recombinant humaan RPRD1B-eiwit

RPRD1B also known as regulation of nuclear pre-mRNA domain-containing protein 1B. This protein interacts with phosphorylated C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit POLR2A, and participates in dephosphorylation of the CTD. It promotes binding of RNA polymerase II to the CCDN1 promoter and to the termination region before the poly-A site but decreases its binding after the poly-A site. It prevents RNA polymerase II from reading through the 3' end termination site and may allow it to be recruited back to the promoter through promotion of the formation of a chromatin loop. It also enhances the transcription of a number of other cell cycle-related genes including CDK2, CDK4, CDK6 and cyclin-E but not CDKN1A, CDKN1B or cyclin-A. It promotes cell proliferation. Recombinant human RPRD1B, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Regulation of nuclear pre-mRNA domain-containing protein 1B, Regulation of nuclear pre-mRNA domain-containing protein 1B, C20orf77, CREPT, dJ1057B20.2, NET60

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

39.3 kDa (349aa) confirmed by MALDI-TOF

RPRD1B, Regulation of nuclear pre-mRNA domain-containing protein 1B, Regulation of nuclear pre-mRNA domain-containing protein 1B, C20orf77, CREPT, dJ1057B20.2, NET60, ATGP3048-10 µg, ATGP3048-20 µg, ATGP3048-50 µg, ATGP3048-100 µg, ATGP3048-250 µg, ATGP3048-500 µg, ATGP3048-1 mg, ATGP3048-10, ATGP3048-20, ATGP3048-50, ATGP3048-100, ATGP3048-250, ATGP3048-500, ATGP3048-1

Lu D., et al. (2012) Cancer Cell 21:92-104

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 20% glycerol

Other

NP_067038.1

Q9NQG5

Human

MSSFSES ALEKKLSELS NSQQSVQTLS LWLIHHRKHA GPIVSVWHRE LRKAKSNRKL TFLYLANDVI QNSKRKGPEF TREFESVLVD AFSHVAREAD EGCKKPLERL LNIWQERSVY GGEFIQQLKL SMEDSKSPPP KATEEKKSLK RTFQQIQEEE DDDYPGSYSP QDPSAGPLLT EELIKALQDL ENAASGDATV RQKIASLPQE VQDVSLLEKI TDKEAAERLS KTVDEACLLL AEYNGRLAAE LEDRRQLARM LVEYTQNQKD VLSEKEKKLE EYKQKLARVT QVRKELKSHI QSLPDLSLLP NVTGGLAPLP SAGDLFSTD