Recombinant humaan RPS10-eiwit

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. RPS10 is a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S10E family of ribosomal proteins. It is located in the cytoplasm. Variable expression of this gene in colorectal cancers compared to adjacent normal tissues has been observed, although no correlation between the level of expression and the severity of the disease has been found. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Recombinant human RPS10 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

40S ribosomal protein S10, DBA9, S10

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

21 kDa (188aa) confirmed by MALDI-TOF

RPS10, 40S ribosomal protein S10, DBA9, S10, ATGP2031-10 µg, ATGP2031-20 µg, ATGP2031-50 µg, ATGP2031-100 µg, ATGP2031-250 µg, ATGP2031-500 µg, ATGP2031-1 mg, ATGP2031-10, ATGP2031-20, ATGP2031-50, ATGP2031-100, ATGP2031-250, ATGP2031-500, ATGP2031-1

Yu Y, Ji H, et al. (2005) . Protein Sci. 14 (6) :1438-46.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 30% glycerol, 1mM DTT

Epigenomics (Transcription & Translation)

NP_001190174

P46783

Human

MGSSHHHHHH SSGLVPRGSH MGSMLMPKKN RIAIYELLFK EGVMVAKKDV HMPKHPELAD KNVPNLHVMK AMQSLKSRGY VKEQFAWRHF YWYLTNEGIQ YLRDYLHLPP EIVPATLRRS RPETGRPRPK GLEGERPARL TRGEADRDTY RRSAVPPGAD KKAEAGAGSA TEFQFRGGFG RGRGQPPQ