Recombinant humaan RPS3-eiwit

40S ribosomal protein S3, also known as RPS3, belongs to the S3P family of ribosomal proteins. RPS3 mediates the formation of the mRNA binding site 3' of the codon in the decoding site. In addition, RPS3 is involved in DNA damage recognition as shown by its affinity for abasic sites and 7, 8-dihydro-8-oxoguanine residues and its interaction with human base excision repair proteins OGG1 and Ref-1. Higher levels of expression of this gene in colon adenocarcinomas and adenomatous polyps compared to adjacent normal colonic mucosa have been observed. Recombinant human RPS3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

40S ribosomal protein S3

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

28.8 kDa (263aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

RPS3, 40S ribosomal protein S3, ATGP1254-10 µg, ATGP1254-20 µg, ATGP1254-50 µg, ATGP1254-100 µg, ATGP1254-250 µg, ATGP1254-500 µg, ATGP1254-1 mg, ATGP1254-10, ATGP1254-20, ATGP1254-50, ATGP1254-100, ATGP1254-250, ATGP1254-500, ATGP1254-1

Hegde V (2006) DNA Repair. 5:810-815.; ; Choi S H., et al. (2006) J Biochem Mol Biol. 39:208-215.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl

Signal Transduction

NP_000996.2

P23396

Human

MAVQISKKRK FVADGIFKAE LNEFLTRELA EDGYSGVEVR VTPTRTEIII LATRTQNVLG EKGRRIRELT AVVQKRFGFP EGSVELYAEK VATRGLCAIA QAESLRYKLL GGLAVRRACY GVLRFIMESG AKGCEVVVSG KLRGQRAKSM KFVDGLMIHS GDPVNYYVDT AVRHVLLRQG VLGIKVKIML PWDPTGKIGP KKPLPDHVSI VEPKDEILPT TPISEQKGGK PEPPAMPQPV PTA