Recombinant humaan RRAGC-eiwit

Ras-related GTP binding C, also known as RRAGC, is a monomeric guanine nucleotide-binding protein, or G protein. By binding GTP or GDP, small G proteins act as molecular switches in numerous cell processes and signaling pathways. RRAGC has intrinsic GTPase activity. It also regulates the organization of the actin cytoskeleton. Recombinant human RRAGC protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Ras-related GTP binding C, GTR2, RAGC

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

46.7 kDa (423aa) confirmed by MALDI-TOF

RRAGC, Ras-related GTP binding C, GTR2, RAGC, ATGP1436-10 µg, ATGP1436-20 µg, ATGP1436-50 µg, ATGP1436-100 µg, ATGP1436-250 µg, ATGP1436-500 µg, ATGP1436-1 mg, ATGP1436-10, ATGP1436-20, ATGP1436-50, ATGP1436-100, ATGP1436-250, ATGP1436-500, ATGP1436-1

Sekiquchi T., et al. (2001) J Biol Chem. 276:7246-57.; ; Sekiquchi T., et al. (2004) J Biol Chem. 279:8343-50.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl

Signal Transduction

NP_071440

Q9HB90

Human

MGSSHHHHHH SSGLVPRGSH MGSHMSLQYG AEETPLAGSY GAADSFPKDF GYGVEEEEEE AAAAGGGVGA GAGGGCGPGG ADSSKPRILL MGLRRSGKSS IQKVVFHKMS PNETLFLEST NKIYKDDISN SSFVNFQIWD FPGQMDFFDP TFDYEMIFRG TGALIYVIDA QDDYMEALTR LHITVSKAYK VNPDMNFEVF IHKVDGLSDD HKIETQRDIH QRANDDLADA GLEKLHLSFY LTSIYDHSIF EAFSKVVQKL IPQLPTLENL LNIFISNSGI EKAFLFDVVS KIYIATDSSP VDMQSYELCC DMIDVVIDVS CIYGLKEDGS GSAYDKESMA IIKLNNTTVL YLKEVTKFLA LVCILREESF ERKGLIDYNF HCFRKAIHEV FEVGVTSHRS CGHQTSASSL KALTHNGTPR NAI