Recombinant humaan RRAS-eiwit

RRAS, also known as ras-related protein R-Ras, belongs to the Ras family which functions in signal transduction pathways. This protein has been shown to interact with RASSF5, NCK1, Bcl-2, ARAF and RALGDS. RRAS can bind GTP and GDP, and it has intrinsic GTPase activity. It also regulates the organization of the actin cytoskeleton. Recombinant RRAS protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Ras-related protein R-Ras, p23, Related RAS viral (r-ras) oncogene homolog, R-Ras, Oncogene RRAS

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

25.3 kDa (235aa) confirmed by MALDI-TOF

RRAS, ATGP0534-10 µg, ATGP0534-20 µg, ATGP0534-50 µg, ATGP0534-100 µg, ATGP0534-250 µg, ATGP0534-500 µg, ATGP0534-1 mg, ATGP0534-10, ATGP0534-20, ATGP0534-50, ATGP0534-100, ATGP0534-250, ATGP0534-500, ATGP0534-1

Lowe DG. (1987) Cell. 48 (1) :137-46.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH8.5) containing 30% glycerol, 0.1M NaCl

Signal Transduction

NP_006261

P10301

Human

MSSGAASGTG RGRPRGGGPG PGDPPPSETH KLVVVGGGGV GKSALTIQFI QSYFVSDYDP TIEDSYTKIC SVDGIPARLD ILDTAGQEEF GAMREQYMRA GHGFLLVFAI NDRQSFNEVG KLFTQILRVK DRDDFPVVLV GNKADLESQR QVPRSEASAF GASHHVAYFE ASAKLRLNVD EAFEQLVRAV RKYQEQELPP SPPSAPRKKG GGCPC