Recombinant humaan RTCA-eiwit

RTCA catalyzes the conversion of 3'-phosphate to a 2', 3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) transfer of adenylate to an RNA-N3'P to produce RNA-N3'PP5'A; (C) and attack of the adjacent 2'-hydroxyl on the 3'-phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing. Recombinant human RTCA protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

RNA 3-terminal phosphate cyclase isoform b, RNA 3'-terminal phosphate cyclase, RPC, RTC1, RTCD1

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

41.7 kDa (389aa) confirmed by MALDI-TOF

RTCA, RNA 3-terminal phosphate cyclase isoform b, RNA 3'-terminal phosphate cyclase, RPC, RTC1, RTCD1, ATGP2735-10 µg, ATGP2735-20 µg, ATGP2735-50 µg, ATGP2735-100 µg, ATGP2735-250 µg, ATGP2735-500 µg, ATGP2735-1 mg, ATGP2735-10, ATGP2735-20, ATGP2735-50, ATGP2735-100, ATGP2735-250, ATGP2735-500, ATGP2735-1

Genschik P., Billy E., et al. (1997) EMBO J. 16:2955-2967

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT

Epigenomics (Transcription & Translation)

NP_003720.1

O00442

Human

MAGPRVE VDGSIMEGGG QILRVSTALS CLLGLPLRVQ KIRAGRSTPG LRPQHLSGLE MIRDLCDGQL EGAEIGSTEI TFTPEKIKGG IHTADTKTAG SVCLLMQVSM PCVLFAASPS ELHLKGGTNA EMAPQIDYTV MVFKPIVEKF GFIFNCDIKT RGYYPKGGGE VIVRMSPVKQ LNPINLTERG CVTKIYGRAF VAGVLPFKVA KDMAAAAVRC IRKEIRDLYV NIQPVQEPKD QAFGNGNGII IIAETSTGCL FAGSSLGKRG VNADKVGIEA AEMLLANLRH GGTVDEYLQD QLIVFMALAN GVSRIKTGPV TLHTQTAIHF AEQIAKAKFI VKKSEDEEDA AKDTYIIECQ GIGMTNPNL