Recombinant humaan RTN4IP1-eiwit

RTN4IP1 is a novel mitochondrial protein that interacts with reticulon 4, which is a potent inhibitor of regeneration following spinal cord injury. The interaction of reticulon 4 with mitochondrial proteins may provide insight into the mechanisms for reticulon-induced inhibition of neurite growth. Recombinant human RTN4IP1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Reticulon-4-interacting protein 1, MGC12934, NIMP, Reticulon 4 interacting protein 1

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

41.4 kDa (379aa) confirmed by MALDI-TOF

RTN4IP1, Reticulon-4-interacting protein 1, MGC12934, NIMP, Reticulon 4 interacting protein 1, ATGP1360-10 µg, ATGP1360-20 µg, ATGP1360-50 µg, ATGP1360-100 µg, ATGP1360-250 µg, ATGP1360-500 µg, ATGP1360-1 mg, ATGP1360-10, ATGP1360-20, ATGP1360-50, ATGP1360-100, ATGP1360-250, ATGP1360-500, ATGP1360-1

Huber A.B. et al. (2000) Biol. Chem. 381: 407-419.; ; Hu W.H. et al. (2002) J. Neurochem. 81: 36-45.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 30% glycerol, 0.1M NaCl

Metabolism

NP_116119

Q8WWV3

Human

MGSSHHHHHH SSGLVPRGSH MGSTVMPAWV IDKYGKNEVL RFTQNMMMPI IHYPNEVIVK VHAASVNPID VNMRSGYGAT ALNMKRDPLH VKIKGEEFPL TLGRDVSGVV MECGLDVKYF KPGDEVWAAV PPWKQGTLSE FVVVSGNEVS HKPKSLTHTQ AASLPYVALT AWSAINKVGG LNDKNCTGKR VLILGASGGV GTFAIQVMKA WDAHVTAVCS QDASELVRKL GADDVIDYKS GSVEEQLKSL KPFDFILDNV GGSTETWAPD FLKKWSGATY VTLVTPFLLN MDRLGIADGM LQTGVTVGSK ALKHFWKGVH YRWAFFMASG PCLDDIAELV DAGKIRPVIE QTFPFSKVPE AFLKVERGHA RGKTVINVV