Recombinant humaan RUNX3/CBFA3-eiwit

RuNX3, also known as runt-related transcription factor 3, belongs to the RuNX family that regulate the expression of genes involved in cellular differentiation and cell cycle progression. A heterodimer of this protein and a beta subunit forms a complex that binds to the core DNA sequence 5'-PYGPYGGT-3' found in a number of enhancers and promoters, and can either activate or suppress transcription. It also interacts with other transcription factors. It is a strong candidate as a gastric cancer tumor suppressor. Recombinant human RuNX3, fused to His-tag at N-terminus, protein was expressed in E. coli and purified by using conventional chromatography techniques

Runt-related transcription factor 3, AML2, CBFA3, PEBP2aC, Runt-related transcription factor 3 Acute myeloid leukemia 2 protein, Acute myeloid leukemia gene 2, AML 2, CBF alpha 3, CBFA 3, Core binding factor alpha 3 subunit, core binding factor, Core binding factor runt domain alpha subunit 3, Core binding factor subunit alpha 3.M574, core-binding factor, FLJ34510, MGC16070, Oncogene AML 2, PEA2 alpha C, PEBP2 alpha C, Pebp2a3, Polyomavirus enhancer binding protein 2 alpha C subunit, runt domain alpha subunit 3, runt related transcription factor 3, RuNX 3, SL3 3 enhancer factor 1 alpha C subunit, SL3/AKV core binding factor alpha C subunit, Transcription factor AML2.

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

17.1 kDa (155aa) confirmed by MALDI-TOF

RUNX3, CBFA3, Runt-related transcription factor 3, AML2, CBFA3, PEBP2aC, Runt-related transcription factor 3 Acute myeloid leukemia 2 protein, Acute myeloid leukemia gene 2, AML 2, CBF alpha 3, CBFA 3, Core binding factor alpha 3 subunit, core binding factor, Core binding factor runt domain alpha subunit 3, Core binding factor subunit alpha 3.M574, core-binding factor, FLJ34510, MGC16070, Oncogene AML 2, PEA2 alpha C, PEBP2 alpha C, Pebp2a3, Polyomavirus enhancer binding protein 2 alpha C subunit, runt domain alpha subunit 3, runt related transcription factor 3, RuNX 3, SL3 3 enhancer factor 1 alpha C subunit, SL3/AKV core binding factor alpha C subunit, Transcription factor AML2., ATGP0576-10 µg, ATGP0576-20 µg, ATGP0576-50 µg, ATGP0576-100 µg, ATGP0576-250 µg, ATGP0576-500 µg, ATGP0576-1 mg, ATGP0576-10, ATGP0576-20, ATGP0576-50, ATGP0576-100, ATGP0576-250, ATGP0576-500, ATGP0576-1

Vogiatzi P. et al. (2006) Cancer Biol Ther. 5 (4) :371-4.; ; Li QL, et al. (2002) Cell. 109 (1) :113-24.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT

Epigenomics (Transcription & Translation)

NP_004341

Q13761

Human

MRSMVDVLAD HAGELVRTDS PNFLCSVLPS HWRCNKTLPV AFKVVALGDV PDGTVVTVMA GNDENYSAEL RNASAVMKNQ VARFNDLRFV GRSGRGKSFT LTITVFTNPT QVATYHRAIK VTVDGPREPR RHRQK