Recombinant humaan RWDD1-eiwit

RWDD1, also known as CGI-24 or PTD013, belongs to the RWDD1/GIR2 family. This protein protects DRG2 (developmentally regulated GTP binding protein 2), from proteolytic degradation. RWDD1 is a novel RWD domain containing protein. RWD domain refers to three signature motifs in proteins: RING finger, WD-repeats, and yeast DEAD (DEXD) -like motif. The function of this domain is not clear enough. Present evidence suggests that RWD domain might be necessary for protein-protein interaction. Recombinant human RWDD1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

RWD domain-containing protein 1, CGI-24, PTD013

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

30.3 kDa (266aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

RWDD1, RWD domain-containing protein 1, CGI-24, PTD013, ATGP1844-10 µg, ATGP1844-20 µg, ATGP1844-50 µg, ATGP1844-100 µg, ATGP1844-250 µg, ATGP1844-500 µg, ATGP1844-1 mg, ATGP1844-10, ATGP1844-20, ATGP1844-50, ATGP1844-100, ATGP1844-250, ATGP1844-500, ATGP1844-1

Kang N, et al. (2008) Cell Mol Immunol. 5 (5) :333-9.; ; Grotsch H, et al. (2012) Mol Cell Endocrinol. 358 (1) :53-62.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl,10% glycerol, 2mM DTT

Protease inhibitors

NP_057036

Q9H446

Human

MTDYGEE QRNELEALES IYPDSFTVLS ENPPSFTITV TSEAGENDET VQTTLKFTYS EKYPDEAPLY EIFSQENLED NDVSDILKLL ALQAEENLGM VMIFTLVTAV QEKLNEIVDQ IKTRREEEKK QKEKEAEEAE KQLFHGTPVT IENFLNWKAK FDAELLEIKK KRMKEEEQAG KNKLSGKQLF ETDHNLDTSD IQFLEDAGNN VEVDESLFQE MDDLELEDDE DDPDYNPADP ESDSAD