Recombinant humaan RYBP-eiwit

RYBP (RING1- and YY1-binding protein) is a member of the polycomb group (PcG) . This protein interacts with MDM2 and decreases MDM2-mediated p53 ubiquitination, leading to stabilization of p53 and an increase in p53 activity. RYBP induces cell-cycle arrest and is involved in the p53 response to DNA damage. RYBP has been shown to interact with RING1, YY1, Caspase 10, E2F3, E2F2, Mdm2, Abl gene and CBX2. Recombinant human RYBP protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

RING1 and YY1 binding protein, AAP1, DEDAF, YEAF1

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

27.4 kDa (252aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

RYBP, RING1 and YY1 binding protein, AAP1, DEDAF, YEAF1, ATGP1600-10 µg, ATGP1600-20 µg, ATGP1600-50 µg, ATGP1600-100 µg, ATGP1600-250 µg, ATGP1600-500 µg, ATGP1600-1 mg, ATGP1600-10, ATGP1600-20, ATGP1600-50, ATGP1600-100, ATGP1600-250, ATGP1600-500, ATGP1600-1

Chen D, et al. (2008) EMBO Rep. 10 (2) :166-72. ; ; Zheng L., et al. (2001) J. Biol. Chem. 276:31945-31952

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 1mM DTT, 100 mM NaCl

Apoptosis

NP_036366

Q8N488

Human

MGSSHHHHHH SSGLVPRGSH MGSHMTMGDK KSPTRPKRQA KPAADEGFWD CSVCTFRNSA EAFKCSICDV RKGTSTRKPR INSQLVAQQV AQQYATPPPP KKEKKEKVEK QDKEKPEKDK EISPSVTKKN TNKKTKPKSD ILKDPPSEAN SIQSANATTK TSETNHTSRP RLKNVDRSTA QQLAVTVGNV TVIITDFKEK TRSSSTSSST VTSSAGSEQQ NQSSSGSEST DKGSSRSSTP KGDMSAVNDE SF