Recombinant humaan S100A5 eiwit

S100 calcium binding protein A5, also known as S100A5, is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100A5 has a Ca2+ affinity 20- to 100-fold higher than the other S100 proteins studied under identical conditions. This protein also binds Zn2+ and Cu2+, and Cu2+ strongly which impairs the binding of Ca2+. S100A5 is expressed in very restricted regions of the adult brain. Recombinant human S100A5 protien, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

S100 calcium binding protein A5, S100D

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

12.9 kDa (112aa) confirmed by MALDI-TOF

S100A5, S100 calcium binding protein A5, S100D, ATGP0957-10 µg, ATGP0957-20 µg, ATGP0957-50 µg, ATGP0957-100 µg, ATGP0957-250 µg, ATGP0957-500 µg, ATGP0957-1 mg, ATGP0957-10, ATGP0957-20, ATGP0957-50, ATGP0957-100, ATGP0957-250, ATGP0957-500, ATGP0957-1

Schafer BW., et al. (1996) Trends Biochem Sci. 21 (4) :134-40.; ; Schafer BW., et al. (2000) J Biol Chem. 275 (39) :30623-30

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 30% glycerol, 0.1M NaCl, 0.1mM PMSF

Signal Transduction

NP_002953

P33763

Human

MGSSHHHHHH SSGLVPRGSH METPLEKALT TMVTTFHKYS GREGSKLTLS RKELKELIKK ELCLGEMKES SIDDLMKSLD KNSDQEIDFK EYSVFLTMLC MAYNDFFLED NK