Recombinant humaan S100A7 eiwit

S100A7, also known as Psoriasin, belongs to the S-100 family of calcium binding proteins and is secreted via a non-classical secretory pathway into the cytoplasm. It is thought to function in the regulation of many cellular processes, including the cell cycle, cell progression and cellular differentiation. It contains two EF-hand domains and is highly upregulated in psoriatic epidermis, as well as in bladder squamous cell carcinoma and breast cancer tissue, suggesting a possible role in carcinogenesis. Recombinant human S100A7 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

S100 calcium binding protein A7, S100A7c, Psoriasin

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

13.6 kDa (121aa) confirmed by MALDI-TOF

S100A7, S100 calcium binding protein A7, S100A7c, Psoriasin, ATGP0991-10 µg, ATGP0991-20 µg, ATGP0991-50 µg, ATGP0991-100 µg, ATGP0991-250 µg, ATGP0991-500 µg, ATGP0991-1 mg, ATGP0991-10, ATGP0991-20, ATGP0991-50, ATGP0991-100, ATGP0991-250, ATGP0991-500, ATGP0991-1

Celis J.E., et al. (1998) . Structure., 6: 477-489; ; Martinsson H., et al. (2005) . Exp. Dermatol., 14: 161-168.

Jung SH, et al. Integrative proteomic profiling of protein activity and interactions using protein arrays. (Mol Cell Proteomics. 2012) {https://pubmed.ncbi.nlm.nih.gov/22843993/}; ; Jung SH, et al. Identification of transglutaminase 2 kinase substrates using a novel on-chip activity assay. (Biosens Bioelectron. 2016) {https://pubmed.ncbi.nlm.nih.gov/27040940/}; ; Jung SH, et al. Systematic investigation of protein kinase A substrate proteins using on-chip protein kinase kinetic profiling. (Analyst. 2017) {https://pubmed.ncbi.nlm.nih.gov/28536714/}

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 30% glycerol, 0.1M NaCl, 1mM DTT

Cancer

AAH34687

P31151

Human

MGSSHHHHHH SSGLVPRGSH MSNTQAERSI IGMIDMFHKY TRRDDKIDKP SLLTMMKENFPNFLSACDKK GTNYLADVFE KKDKNEDKKI DFSEFLSLLG DIATDYHKQS HGAAPCSGGS Q