Recombinant humaan S6K1/RPS6KB1-eiwit

RPS6KB1, also known as ribosomal protein S6 kinase beta-1 isoform a, is a serine/threonine kinase that acts downstream of PIP3 and phosphoinositide-dependent kinase-1 in the PI3 kinase pathway. Phosphorylation of S6 induces protein synthesis at the ribosome. The phosphorylation of p70S6K at threonine 389 has been used as a hallmark of activation by mTOR and correlated with autophagy inhibition in various situations. However, several recent studies suggest that the activity of p70S6K plays a more positive role in the increase of autophagy. Recombinant human RPS6KB1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

STK14A, Serine/threonine-protein kinase 14A, S6K-beta-1, S6K1, S6K, RPS6KB1, Ribosomal protein S6 kinase I, Ribosomal protein S6 kinase beta-1 isoform a, PS6K, P70S6K1, p70-S6K, p70-alpha, p70 (S6K) -alpha, p70 S6KA, p70 S6 kinase alpha, p70 ribosomal S6 kinase alpha, 70 kDa ribosomal protein S6 kinase 1

Baculovirus

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

< 1 EU per 1ug of protein (determined by LAL method)

> 85% by SDS-PAGE

60.2 kDa (533aa)

RPS6KB1, Ribosomal protein S6 kinase beta-1 isoform a, RPS6KB1, p70 S6KA, p70 (S6K) -alpha, p70-alpha, p70-S6K, PS6K, S6K, S6K-beta-1, S6K1, STK14A, ATGP3741-10 µg, ATGP3741-20 µg, ATGP3741-50 µg, ATGP3741-100 µg, ATGP3741-250 µg, ATGP3741-500 µg, ATGP3741-1 mg, ATGP3741-10, ATGP3741-20, ATGP3741-50, ATGP3741-100, ATGP3741-250, ATGP3741-500, ATGP3741-1

Grove JR., et al, (1991) Mol Cell Biol. 11:5541-5550.; ; Ferrari S., et al, (1994) Crit Rev Biochem Mol Biol. 29:385-413.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 50 mM Tris-HCl buffer (pH 8.0) containing 40% glycerol, 0.5M NaCl, 2mM DTT, 0.1mM PMSF

Epigenomics (Transcription & Translation)

NP_003152

P23443

Human

Met1