Recombinant humaan SAE2/UBA2-eiwit

UBA2, also known as SUMO-activating enzyme subunit 2, is a family of small and related proteins that can be enzymatically attached to a target protein by a post-translational modification process termed sumoylation. It is conjugated to a variety of molecules in the presence of the SAE1/UBA2 SUMO-activating (E1) enzyme and the UBE2I/Ubc9 SUMO-conjugating (E2) enzyme. It may represent an important mechanism to protect neurons during episodes of cerebral ischemia. Recombinant human UBA2, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Ubiquitin like modifier activating enzyme 2, SUMO1 activating enzyme subunit 2, SUMO-activating enzyme subunit 2, Anthracycline-associated resistance ARX, Ubiquitin-like 1-activating enzyme E1B, Ubiquitin-like modifier-activating enzyme 2, Ubiquitin-activating enzyme E1 homolog, UBLE1B

Baculovirus

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 85% by SDS-PAGE

72.3 kDa (649aa)

UBA2, SUMO-activating enzyme subunit 2, UBA2, ARX, HRIHFB2115, SAE2, ATGP3363-10 µg, ATGP3363-20 µg, ATGP3363-50 µg, ATGP3363-100 µg, ATGP3363-250 µg, ATGP3363-500 µg, ATGP3363-1 mg, ATGP3363-10, ATGP3363-20, ATGP3363-50, ATGP3363-100, ATGP3363-250, ATGP3363-500, ATGP3363-1

Gong L., et al. (1999) FEBS Lett. 448:185-189.; ; Tatham MH., et al. (2001) J Biol Chem. 276:35368-35374.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Ubiquitination

NP_005490.1

Q9UBT2

Human

Ala

MALSRGL PRELAEAVAG GRVLVVGAGG IGCELLKNLV LTGFSHIDLI DLDTIDVSNL NRQFLFQKKH VGRSKAQVAK ESVLQFYPKA NIVAYHDSIM NPDYNVEFFR QFILVMNALD NRAARNHVNR MCLAADVPLI ESGTAGYLGQ VTTIKKGVTE CYECHPKPTQ RTFPGCTIRN TPSEPIHCIV WAKYLFNQLF GEEDADQEVS PDRADPEAAW EPTEAEARAR ASNEDGDIKR ISTKEWAKST GYDPVKLFTK LFKDDIRYLL TMDKLWRKRK PPVPLDWAEV QSQGEETNAS DQQNEPQLGL KDQQVLDVKS YARLFSKSIE TLRVHLAEKG DGAELIWDKD DPSAMDFVTS AANLRMHIFS MNMKSRFDIK SMAGNIIPAI ATTNAVIAGL IVLEGLKILS GKIDQCRTIF LNKQPNPRKK LLVPCALDPP NPNCYVCASK PEVTVRLNVH KVTVLTLQDK IVKEKFAMVA PDVQIEDGKG TILISSEEGE TEANNHKKLS EFGIRNGSRL QADDFLQDYT LLINILHSED LGKDVEFEVV GDAPEKVGPK QAEDAAKSIT NGSDDGAQPS TSTAQEQDDV LIVDSDEEDS SNNADVSEEE RSRKRKLDEK ENLSAKRSRI EQKEELDDVI ALD