Recombinant humaan SAR1A-eiwit

SAR1a gene homolog 1, also known as SAR1A, belongs to the small GTPase superfamily. Sar1 is an essential component of COPII vesicle coats involved in export of cargo from the ER. The GTPase activity of Sar1 functions as a molecular switch to control protein-protein and protein-lipid interactions that direct vesicle budding from the ER. GDP-bound Sar1 interacts with the membrane-bound exchange factor Sec12 and exchanges its bound GDP for GTP. Recombinant human SAR1A, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

SARA 1, Sara, SAR1a gene homolog 1 COPII associated small GTPase, SAR1a gene homolog 1 (S. cerevisiae), SAR1A, SAR1 homolog A (S. cerevisiae), SAR1 gene homolog A, SAR 1, Masra 2, GTP binding protein SAR1a, COPII-associated small GTPase

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

24.5 kDa (218aa) confirmed by MALDI-TOF

COPII-associated small GTPase, GTP binding protein SAR1a, Masra 2, Masra2, SAR 1, SAR1, SAR1 gene homolog A, SAR1 homolog A (S. cerevisiae), SAR1A, SAR1a gene homolog (S cerevisiae) 1, SAR1a gene homolog (S. cerevisiae) 1, SAR1a gene homolog 1, SAR1a gene homolog 1 (S cerevisiae), SAR1a gene homolog 1 (S. cerevisiae), SAR1a gene homolog 1 COPII associated small GTPase, Sara, SARA 1, SARA1, ATGP0347-10 µg, ATGP0347-20 µg, ATGP0347-50 µg, ATGP0347-100 µg, ATGP0347-250 µg, ATGP0347-500 µg, ATGP0347-1 mg, ATGP0347-010, ATGP0347-020, ATGP0347-050, ATGP0347-100, ATGP0347-250, ATGP0347-500, ATGP0347-01M

Jones B., et al. (2003) Nat Genet. 34 (1) :29-31. ; ; Bi X., et al. (2007) Dev Cell. 13 (5) :635-45. ;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol

Signal Transduction

NP_064535

Q9NR31

Human

MSFIFEWIYN GFSSVLQFLG LYKKSGKLVF LGLDNAGKTT LLHMLKDDRL GQHVPTLHPT SEELTIAGMT FTTFDLGGHE QARRVWKNYL PAINGIVFLV DCADHSRLVE SKVELNALMT DETISNVPIL ILGNKIDRTD AISEEKLREI FGLYGQTTGK GNVTLKELNA RPMEVFMCSV LKRQGYGEGF RWLSQYID