Recombinant humaan SARS-eiwit

SARS (Seryl-tRNA synthetase, cytoplasmic) belongs to the class-II aminoacyl-tRNA synthetase family. Aminoacyl-tRNA synthetases function to catalyze the aminoacylation of tRNAs by their corresponding amino acids, thus linking amino acids with tRNA-contained nucleotide triplets. This enzyme catalyzes the attachment of serine to tRNA (Ser) . It is probably able to aminoacylate tRNA (Sec) with serine, to form the misacylated tRNA L-seryl-tRNA (Sec), which will be further converted into selenocysteinyl-tRNA (Sec) . Recombinant human SARS protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Seryl-tRNA synthetase, SERRS, SERS

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

61.2 kDa (537aa)

SARS, Seryl-tRNA synthetase, SERRS, SERS, Sars-CoV-2 S1, Sars-CoV-2 S1, covid, corona, ATGP1357-10 µg, ATGP1357-20 µg, ATGP1357-50 µg, ATGP1357-100 µg, ATGP1357-250 µg, ATGP1357-500 µg, ATGP1357-1 mg, ATGP1357-10, ATGP1357-20, ATGP1357-50, ATGP1357-100, ATGP1357-250, ATGP1357-500, ATGP1357-1

Hartlein M, et al. (1995) J. Mol. Evol. 40 (5) : 519-30.; ; Vincent C., et al. (1997) Eur. J. Biochem. 250:77-84

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 100 mM NaCl

Metabolism

NP_006504

P49591

Human

MVLDLDL FRVDKGGDPA LIRETQEKRF KDPGLVDQLV KADSEWRRCR FRADNLNKLK NLCSKTIGEK MKKKEPVGDD ESVPENVLSF DDLTADALAN LKVSQIKKVR LLIDEAILKC DAERIKLEAE RFENLREIGN LLHPSVPISN DEDVDNKVER IWGDCTVRKK YSHVDLVVMV DGFEGEKGAV VAGSRGYFLK GVLVFLEQAL IQYALRTLGS RGYIPIYTPF FMRKEVMQEV AQLSQFDEEL YKVIGKGSEK SDDNSYDEKY LIATSEQPIA ALHRDEWLRP EDLPIKYAGL STCFRQEVGS HGRDTRGIFR VHQFEKIEQF VYSSPHDNKS WEMFEEMITT AEEFYQSLGI PYHIVNIVSG SLNHAASKKL DLEAWFPGSG AFRELVSCSN CTDYQARRLR IRYGQTKKMM DKVEFVHMLN ATMCATTRTI CAILENYQTE KGITVPEKLK EFMPPGLQEL IPFVKPAPIE QEPSKKQKKQ HEGSKKKAAA RDVTLENRLQ NMEVTDA