Recombinant humaan SAT1-eiwit

Diamine N-acetyltransferase 1 (or Spermidine/spermine-N1-acetyltransferase, SSAT1), as known as SAT-1, is a polyamine catabolic enzyme which catalyzes the acetylation of polyamines. This protein plays an important role in polyamine homoeostasis, since acetylated products are either excreted from the cell or oxidized by acetylpolyamine oxidase. A variety of other effects of increased SAT-1 activity include death of pancreatic cells, blockage of regenerative tissue growth, behavioral changes, keratosis follicularis spinulosa decalvans (KFSD), and hair loss. Recombinant human SAT-1, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Spermidine/spermine N1-acetyltransferase 1, DC21, KFSD, KFSDX, SAT, SSAT, SSAT-1, SAT1, Diamine acetyltransferase 1, EC 2.3.1.57, Polyamine N-acetyltransferase 1, Putrescine acetyltransferase, Spermidine/spermine N (1) -acetyltransferase 1, SSAT 1.

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

22.1 kDa (191aa) confirmed by MALDI-TOF

Diamine N-acetyltransferase1, spermidine/spermine N1-acetyltransferase 1, DC21, KFSD, KFSDX, SAT, SSAT, SSAT-1, SAT1, Diamine acetyltransferase 1, EC 2.3.1.57, Polyamine N-acetyltransferase 1, Putrescine acetyltransferase, Spermidine/spermine N (1) -acetyltransferase 1, SSAT 1., ATGP0364-10 µg, ATGP0364-20 µg, ATGP0364-50 µg, ATGP0364-100 µg, ATGP0364-250 µg, ATGP0364-500 µg, ATGP0364-1 mg, ATGP0364-10, ATGP0364-20, ATGP0364-50, ATGP0364-100, ATGP0364-250, ATGP0364-500, ATGP0364-1

Pegg AE., et al. (2008) . Am J Physiol Endocrinol Metab. 294: E995-E1010, ; ; Janne J., et al. (2006) . J Cell Mol Med. 10 (4) :933-45.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing, 10% glycerol

Enzymes & Proteases

NP_002961

P21673

Human

MAKFVIRPAT AADCSDILRL IKELAKYEYM EEQVILTEKD LLEDGFGEHP FYHCLVAEVP KEHWTPEGHS IVGFAMYYFT YDPWIGKLLY LEDFFVMSDY RGFGIGSEIL KNLSQVAMRC RCSSMHFLVA EWNEPSINFY KRRGASDLSS EEGWRLFKID KEYLLKMATE E